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- ISSN: 1389-2010
- E-ISSN: 1873-4316
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Urea-Gradient Protein Refolding in Size Exclusion Chromatography
- Source: Current Pharmaceutical Biotechnology, Volume 11, Issue 3, Apr 2010, p. 289 - 292
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- 01 Apr 2010
Abstract
Protein refolding using urea gradient size exclusion chromatography (UGSEC) is a new version of conventional SEC refolding, which incorporates urea gradient into the SEC. Operating factors, mainly the urea gradient length and the final urea concentration in the gradient, are discussed in detail. Recent applications of UGSEC, including refolding of recombinant human granulocyte colony stimulating factor by UGSEC, is shown as an example.
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