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- ISSN: 1389-2010
- E-ISSN: 1873-4316
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Step-Wise Refolding of Recombinant Proteins
- Source: Current Pharmaceutical Biotechnology, Volume 11, Issue 3, Apr 2010, p. 285 - 288
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- 01 Apr 2010
Abstract
Protein refolding is still on trial-and-error basis. Here we describe step-wise dialysis refolding, in which denaturant concentration is altered in step-wise fashion. This technology controls the folding pathway by adjusting the concentrations of the denaturant and other solvent additives to induce sequential folding or disulfide formation.
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