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image of In vitro Investigation of Antimicrobial and Antioxidant Properties of Green Silver Nanoparticles Synthesized Using Ephedra gerardiana Plant Extract

Abstract

Background

The increasing prevalence of antibiotic-resistant bacteria necessitates exploring nanotechnology as a potential solution for microbial elimination.

Objectives

This study aimed to investigate the antimicrobial and antioxidant effects of silver nanoparticles synthesized using aqueous extract from the () plant (EG@AgNPs).

Methods

Optimal synthesis conditions, including silver nitrate concentration, time, and temperature, were determined. Characterization of EG@AgNPs was conducted, which was followed by antimicrobial assessment against eight bacterial strains and one fungal strain. Additionally, the antioxidant properties of EG@AgNPs were evaluated using the DPPH method.

Results

XRD analysis confirmed EG@AgNPs synthesis. DLS analysis revealed a hydrodynamic diameter of 22 nm. FT-IR analysis confirmed the presence of functional groups from the plant extract in EG@AgNPs. FESEM and TEM images depicted spherical nanoparticles ranging in size from 10 to 20 nm. Antimicrobial investigations using the broth microdilution method demonstrated that plant extract at 7.5 mg/ml inhibited only and growth, with no antimicrobial effects observed at lower concentrations. However, EG@AgNPs significantly enhanced the antimicrobial properties of the plant extract. Notably, these nanoparticles exhibited the most significant effect on and the least on , with MIC value of 125 and 2000 µg/ml, respectively. Furthermore, they inhibited growth at a concentration of 62.5 μg/ml. An assessment of the antioxidant properties of EG@AgNPs indicated a significant increase in antioxidant activity.

Conclusion

The plant extract has emerged as a promising option for silver nanoparticle synthesis. These nanoparticles have been found to exhibit potent antimicrobial properties against Gram-positive and Gram-negative bacterial species, as well as . Additionally, they have demonstrated antioxidant properties.

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2025-01-06
2025-03-30

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/content/journals/cpb/10.2174/0113892010349133241120075750
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In vitro Investigation of Antimicrobial and Antioxidant Properties of Green Silver Nanoparticles Synthesized Using Ephedra gerardiana Plant Extract
Bentham Science Publishers ; https://doi.org/10.2174/0113892010349133241120075750
/content/journals/cpb/10.2174/0113892010349133241120075750
/content/journals/cpb/10.2174/0113892010349133241120075750
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  • Article Type:     Research Article
Keywords: nanoparticles ; antioxidant ; antibacterial ; Ephedra gerardiana ; antifungal ; Silver

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