Current Pharmaceutical Analysis - Volume 5, Issue 4, 2009

The majority of enantiomeric separations for purity analysis and quality control continue to be performed by normal phase liquid chromatography (LC) and supercritical fluid chromatography (SFC). Yet, analysts perform over ninety percent of their routine work using reversed phase systems. When given the opportunity, reversed phase chiral methods are preferred by analysts for they do not require the complex system rinses Read More

Nano-Drug Delivery Systems have emerged as an effective means of targeting therapeutic agents such as drugs, peptides and proteins to various target sites. The unique advantages and potential targeting capability of this approach have claimed it as the drug delivery system of the future. Various analytical methodologies have been used for the evaluation of this delivery system both in vitro and in vivo. This review will atte Read More

Generally, some drugs are present in our body for quite long time at nano or femto gram level and accumulate in body tissues, causing many side effects. Therefore, in the absence of the techniques capable to detect at nano or low level the absence of these drug residues is assumed. Besides, small biological samples such as blood of infants, some hormones and enzymes in our body need detection techniques of nano levels. P Read More

In the past decade, monolithic columns consisting of one piece of an organic polymer or silica with flowthrough pores have been reported in the literature. These materials provide better stability and more important, higher performance than conventional columns packed with particles. This paper summarizes recent applications of monolithic stationary phase in the liquid phase separation, including the separation of enantio Read More

The combination of flexible and robust immunoassay technology with the sensitivity of DNA as a signal amplification template is realized in Immuno-PCR (“IPCR”). Classical ELISA is converted to IPCR by exchange of antibodyenzyme detection conjugates with antibody-DNA conjugates. The value of ultra-sensitive analytics deriving from this for pharmaceutical R&D is threefold: (I) Deeper understanding of biomolecular in Read More

New chromatographic-densitometric method for determination of clopamide and impurities (4-chlorobenzoic and 4-chloro-3-sulfamoylbenzoic acids) in tablets was developed. Silica gel TLC plates as a stationary phase and nbutanol- 2-propanol -water- methylene chloride (10:7:2:5:3 v/v/v/v/v) as a mobile phase were used for separation. Densitometric measurements were done at λ = 235 nm. Favourable retention par Read More

The elecrochemical behavior of ornidazole was investigated using various voltammetric methods. The number of electrons transferred and diffusion coefficient were calculated by using cyclic voltammetry and bulk electrolysis techniques. It is determined that ornidazole is adsorbed on the hanging mercury drop electrode (HMDE) by using cyclic voltammetric data. Therefore, sensitive adsorptive stripping voltammetric Read More

Clopidogrel, being a potent platelet aggregation inhibitor, is used widely around the world to reduce cardiovascular risks in patients with stroke, myocardial infarction, and atherosclerosis. The aim of this review firstly focuses on a comprehensive update of chromatography determination of clopidogrel and its metabolites as well as in human plasma, Wistar rat plasma, and in pharmaceutical preparations. It has been describe Read More

In this work, a simple spectrophotometric methodology based on the competitive aggregation in a dyesurfactant- drug system is described for the determination of pharmaceutical compounds. This is a modification of recently established surfactant to dye binding degree method. Eriochrome Blue Black R (EBBR) and Didodecyldimethylammonium bromide (DDABr) were the dye and surfactant used, respectively. In the propose Read More

Resistance is also an increasing problem for antibiotic based therapy of Helicobacter Pylori infection. A paradigm shift in the treatment of infectious disease may be useful to prevent the further emergence of antibiotic resistance. Some daily-consumed food and beverages, including tea, lactobacillus, alcohol and garlic, have anti-helicobacter activities in-vitro. In epidemiological studies consumption of tea was associated Read More