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2000
Volume 20, Issue 9
  • ISSN: 1573-4129
  • E-ISSN: 1875-676X

Abstract

Background

(Zhi-Zi) is the dry ripe fruit of the plant Ellis (Rubiaceae), which can be used as both food and medicine. Geniposide, a key constituent of , serves as an indicator component for evaluating the quality of . Traditionally, the quantification of geniposide in is achieved through High-performance Liquid Chromatography (HPLC)-based methods.

Objectives

The present study aimed to introduce a rapid approach to quantifying geniposide content in along with validating its effectiveness.

Methods

The experiments involved finding a suitable deuterium solvent, Internal Standard (IS), specific peak, and Nuclear Magnetic Resonance (NMR) parameters for quantitation, and validating specificity, accuracy, precision, and stability.

Results

The results have indicated that methanol- as a solvent has facilitated excellent signal separation in the proton Nuclear Magnetic Resonance (1H NMR) spectroscopy, with trimethyl 1,3,5-benzenetricarboxylate emerging as the ideal IS. The specific signal at δ 7.45, corresponding to H-3 in geniposide, has been identified as the optimal peak for integration. The application of distinctive signals from the 1H NMR spectroscopy has allowed for the precise quantification of geniposide in .

Conclusion

This study has suggested using 1H NMR to quantify geniposide in to be a viable alternative to HPLC-based methods, providing a suitable approach for quality control of .

© 2024 Bentham Science Publishers
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Application of Quantitative Proton Nuclear Magnetic Resonance Spectroscopy for Determination of the Content of Geniposide in Gardeniae fructus
Current Pharmaceutical Analysis 20, 1066 (2024); https://doi.org/10.2174/0115734129352488241105104605
/content/journals/cpa/10.2174/0115734129352488241105104605
/content/journals/cpa/10.2174/0115734129352488241105104605
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  • Article Type:     Research Article
Keyword(s): Gardeniae fructus; geniposide; proton nuclear magnetic resonance; quality control; quantitative determination; validation

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