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2000
Volume 20, Issue 3
  • ISSN: 1573-4129
  • E-ISSN: 1875-676X

Abstract

Background: species known as "Sage" are among the important aromatic plants used in the world. This study, it was investigated the antioxidant capacity of and to investigate its protective effect on oxidative damage in t-BHP-induced fibroblast cells. Methods: Antioxidant activity and phenolic characterization of the extract were evaluated using DPPH, TPC, TFC, FRAP, and HPLC, respectively.TAS, TOS, MDA and 8-oxo-guanine, CAT, SOD, and GPx values were examined using enzyme-linked immunosorbent assay (ELISA). The antiproliferative and apoptosis effects of Salvia cadmica ethanolic extract were determined using XTT assay and fluorescent probes in fibroblast cells. Results: As a result of GC-MS analysis of Salvia cadmica ethanolic extract, carvacrol content was found to be high. The IC value of the DPPH antioxidant assay of the Salvia cadmica ethanolic extract was 80 ± 0.51 μg/mL. TPC, TFC, and FRAP values were found to be 18.25 ± 0.64 (mg gallic acid/g powder), 1.691±0.314 (mg quercetin /g powder), 31.5 ± 0.10 (mg Trolox/g powder), respectively. Total antioxidant and TOS values were found to be 0.383±0.033 (mmol Trolox Equ L-1), 16.31±0.71 (μmol HO L-1) for 0.25 mg/mL, and 0.725±0.05 (mmol Trolox Equ L-1), 12.02 ±0.56 (μmol HO L-1) for 0.5 mg/mL. In addition, while CAT and GPx significantly decreased enzyme activities, no significant change was observed in SOD enzyme activity. extract exhibited apoptotic features compared to only the t-BHP group. Conclusion: These results suggest that extract works through a free radical mechanism.

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/content/journals/cpa/10.2174/0115734129293569240327093703
2024-03-01
2025-07-11
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  • Article Type:
    Research Article
Keyword(s): 8-oxoguanidine; CAT; GPx; phytochemicals; Salvia cadmica; SOD
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