Spectroscopic Studies on Molecular Recognition of Modified Cyclodextrins

This review describes the spectroscopic studies on molecular recognition of a wide variety of guest molecules with native and modified cyclodextrins, including monomodified cyclodextrins, and functional group-bridged bis(cyclodextrin)s. Steady-state and time-resolved fluorescence spectroscopy, circular dichroism spectroscopy, UV-vis spectroscopy, and two-dimensional NMR spectroscopy can be taken to determine the absolute conformation and inclusion complexation behavior of native and modified cyclodextrins. To investigate quantitatively molecular recognition of modified cyclodextrins, different spectrophotometric titration and / or competitive inclusion approach are used to determine the binding constant of inclusion complexes of various guest molecules with native and modified cyclodextrins.