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image of ESM-1 Promotes the Process of Diabetic Nephropathy by Promoting the Expression of CXCL3

Abstract

Background

The analysis of diabetic nephropathy (DN)-related gene dataset demonstrated that C-X-C motif chemokine ligand 3 () is highly expressed in DN. Exploring the impact of in the course of DN is the core goal of this study.

Method

The cell model used in this study was CIHP-1 cells induced by high glucose (HG). qRT-PCR and western blot analysis were carried out to determine the expression difference of CXCL3. After down-regulating the level, we analyzed HG-induced CIHP-1 cell viability by MTT assay, proliferation by EdU staining, apoptosis by flow cytometry, and changes in related protein expression by western blot. In order to analyze the possible regulatory relationship between endothelial cell-specific molecule 1 () and in DN, we constructed an over-expressed plasmid and carried out a rescue experiment.

Result

and were highly expressed in HG-induced podocytes (<0.05). Silenced (si) increased the viability and proliferation of CIHP-1 cells induced by HG, reduced the proportion of apoptosis, and produced corresponding protein changes (<0.01). After the overexpression of , the effects of si were partially offset (<0.05).

Conclusion

In this study, increased HG-induced podocyte damage by promoting expression.

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2025-03-05
2025-05-12

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ESM-1 Promotes the Process of Diabetic Nephropathy by Promoting the Expression of CXCL3
Bentham Science Publishers ; https://doi.org/10.2174/0115665240304670250108055944
/content/journals/cmm/10.2174/0115665240304670250108055944
/content/journals/cmm/10.2174/0115665240304670250108055944
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  • Article Type:     Research Article
Keywords: C-X-C motif chemokine ligand 3 ; podocyte ; endothelial cell-specific molecule 1 ; Diabetic nephropathy

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