- Home
- A-Z Publications
- Current Gene Therapy
- Previous Issues
- Volume 11, Issue 5, 2011
Current Gene Therapy - Volume 11, Issue 5, 2011
Volume 11, Issue 5, 2011
-
-
Editorial [Hot Topic: Recombinase Technology for Gene Therapy (Guest Editor: Zoltan Ivics)]
By Zoltan IvicsThe ability to efficiently deliver foreign genes into cells offers opportunities to use gene therapy to correct genetic diseases. Effective gene therapy requires robust delivery of the desired genes into the relevant target cells, long-term gene expression and minimal risks of secondary effects. DNA recombinases, enzymes that catalyze recombination between DNA molecules, can be harnessed for stable genomic insertion of therape Read More
-
-
-
Targeting the Central Nervous System with Herpes Simplex Virus / Sleeping Beauty Hybrid Amplicon Vectors
Authors: Suresh de Silva and William J. BowersThe pursuits of sustainable treatments for diseases and disorders that afflict the central nervous system (CNS) have proven challenging for the field of viral vector-based gene therapy. However, recent advances in viral vector technology coupled with efficient delivery methods have opened up new avenues that show promise at the preclinical testing stage. The development of the Herpes Simplex Virus/Sleeping Beauty (HSV/SB) Read More
-
-
-
Efficacy and Safety of Sleeping Beauty Transposon-Mediated Gene Transfer in Preclinical Animal Studies
Sleeping Beauty (SB) transposons have been effective in delivering therapeutic genes to treat certain diseases in mice. Hydrodynamic gene delivery of integrating transposons to 5-20% of the hepatocytes in a mouse results in persistent elevated expression of the therapeutic polypeptides that can be secreted into the blood for activity throughout the animal. An alternative route of delivery is ex vivo transformation with SB t Read More
-
-
-
Integrase-Defective Lentiviral Vectors - A Stage for Nonviral Integration Machineries
Authors: Nicklas H. Staunstrup and Jacob G. MikkelsenGene vehicles derived from lentiviruses have become highly esteemed tools for gene transfer and genomic insertion in a wealth of cell types both in vivo and ex vivo. However, accumulating evidence of preferred insertion into actively transcribed genes, driven by biological properties of the parental human immunodeficiency virus type 1, has questioned the safety of this vector technology. As a consequence, integrase-defect Read More
-
-
-
Development of Adenovirus Hybrid Vectors for Sleeping Beauty Transposition in Large Mammals
The Sleeping Beauty (SB) transposase system for somatic integration offers great potential for in vivo gene therapeutic applications and genome engineering. Until recently, however, efficacy of SB transposase as a gene transfer vector especially in large animals was lacking. Herein, we report about the newest viral vector development for delivery of the SB transposase system into large mammals. Over the past decade variou Read More
-
-
-
Therapeutic Applications of the PhiC31 Integrase System
Authors: Christopher L. Chavez and Michele P. CalosThe potential use of the φC31 integrase system in gene therapy opens up the possibilities of new treatments for old diseases. φC31 integrase mediates the integration of plasmid DNA into the chromsomes of mammalian cells in a sequence- specific manner, resulting in robust, long-term transgene expression. In this article, we review how φC31 integrase mediates transgene integration into the genomes of target cells and Read More
-
-
-
Viral and Non-Viral Approaches for Transient Delivery of mRNA and Proteins
Authors: Juliane W. Schott, Melanie Galla, Tamaryin Godinho, Christopher Baum and Axel SchambachThe transient delivery of gene products (RNA or proteins) is not a biotechnological invention but rather an evolutionarily conserved process underlying and regulating a variety of biological functions. On the basis of insights into the underlying mechanisms, several viral and cell-based approaches have been developed for the delivery of RNA or proteins. Prominent applications include the induction of major biological or Read More
-
-
-
Site-Specific Integration by the Adeno-Associated Virus Rep Protein
Authors: Alessandra Recchia and Fulvio MavilioInserting genetic information at precise locations into the human genome has been the goal of gene transfer technology for almost two decades. The spectacular progress of mammalian genetics has led to the development of technology for genome editing and homologous recombination in human somatic cells that is finally approaching efficiency compatible with clinical application. Site-specific integration, or the insertion of Read More
-
-
-
Transposon-Mediated Gene Transfer into Adult and Induced Pluripotent Stem Cells
Authors: Eyayu Belay, Sumitava Dastidar, Thierry VandenDriessche and Marinee K.L. ChuahTransposon technology is a particularly attractive non-viral gene delivery paradigm that allows for efficient genomic integration into a variety of different cell types. In particular, transposon-mediated gene transfer is a promising tool for stem cell research, by virtue of its ability to efficiently and stably transfer genes into adult and induced pluripotent stem (iPS) cells. Moreover, transposons open up new perspectives for non-v Read More
-
-
-
Design and Pre-Clinical Development of Epitope-based DNA Vaccines Against B-Cell Lymphoma
Authors: Sandra Iurescia, Daniela Fioretti, Vito Michele Fazio and Monica RinaldiOptimally designed cancer vaccines should combine the best tumor antigens with the most effective immunotherapy agents and delivery strategies to achieve positive clinical results. The unique immunoglobulin (Ig) idiotype on the surface of each B-cell lymphoma represents an ideal tumor-specific antigen for use as a cancer vaccine. It has been theorized that effective cancer vaccines can be developed using the minimum Read More
-
-
-
Local Gene Delivery for Cancer Therapy
More LessGene therapy is an emerging technique with widespread applications in treatment of cardiovascular diseases, monogenic disorder, infectious diseases, and especially cancers. The major challenge for gene therapy is to deliver therapeutic genes to target tissues. Although various gene delivery vectors such as harmless viruses and micro/nano-particles have been developed (i.e. commonly system delivery), concerns re Read More
-
Volumes & issues
-
Volume 25 (2025)
-
Volume 24 (2024)
-
Volume 23 (2023)
-
Volume 22 (2022)
-
Volume 21 (2021)
-
Volume 20 (2020)
-
Volume 19 (2019)
-
Volume 18 (2018)
-
Volume 17 (2017)
-
Volume 16 (2016)
-
Volume 15 (2015)
-
Volume 14 (2014)
-
Volume 13 (2013)
-
Volume 12 (2012)
-
Volume 11 (2011)
-
Volume 10 (2010)
-
Volume 9 (2009)
-
Volume 8 (2008)
-
Volume 7 (2007)
-
Volume 6 (2006)
-
Volume 5 (2005)
-
Volume 4 (2004)
-
Volume 3 (2003)
-
Volume 2 (2002)
-
Volume 1 (2001)
Most Read This Month
Article
content/journals/cgt
Journal
10
5
false
en

Most Cited Most Cited RSS feed
-
-
New Hope for Intervertebral Disc Degeneration: Bone Marrow Mesenchymal Stem Cells and Exosomes Derived from Bone Marrow Mesenchymal Stem Cell Transplantation
Authors: Xiao-bo Zhang, Xiang-yi Chen, Jin Qi, Hai-yu Zhou, Xiao-bing Zhao, Yi-cun Hu, Rui-hao Zhang, De-chen Yu, Xi-dan Gao, Ke-ping Wang and Lin Ma
-
- More Less