Skip to content
2000
Volume 10, Issue 6
  • ISSN: 1566-5232
  • E-ISSN: 1875-5631

Abstract

Retroviral vectors are presently amongst the most widely used vectors in gene therapy clinical trials to target pathologies of different origins, such as cancers, genetic diseases or neurological disorders. This review provides an overview on the evolution of retroviral vector design and production for gene therapy applications, including state of the art developments in flexible producer cells and safe vectors. In addition, production and purification processes will be addressed, with a particular focus on the improvements undertaken to increase vector productivity and to reduce the rapid loss of infectivity, which presently represent the main challenges in retroviral vectors production for gene therapy.

Loading

Article metrics loading...

/content/journals/cgt/10.2174/156652310793797739
2010-12-01
2024-12-23
Loading full text...

Full text loading...

/content/journals/cgt/10.2174/156652310793797739
Loading

  • Article Type:
    Research Article
Keyword(s): 293 FLEX; 293-SPA; adenosine deaminase deficiency (ADA); AM; Anti-PSMA-Zeta T cell receptor; ATMI/Guerin; avian; biotin-streptavidin bridges; CD19-specific Zeta T cell receptor; CD34 cells; CEM FLY; CRIP; Cronic Granulatomous disease; cryoprotectants; E. coli cytosine deaminase; env (envelope); envelope glycoproteins; feline; Flp293; FLY A4; FLY RD18; gag (group specific antigen); galactosyl(1-3)galactosyl carbohydrate moieties; Gaucher disease; GE, Sartorius; glioblastoma; Glioblastoma; glucocerebrosidades gene; Gp envAm12; Gp91 phox gene; HAII; hematopoetic stem cells; Hemophilia A; HER2-neu-Specific scFvFc-Zeta T Cell Receptor; HIV infection; Hyclone/Thermo, Excellerex; hypercholesterolemia; integrase (IN); IVIM assay; low-density lipoprotein receptor; LTRs (long terminal repeats); MA, CA; Matrix (MA) proteins; melanom; Metastic Breast Cancer; MLV LTR; MLV SIN vectors; Moloney Murine Leukemia Viruses; Multiple Sclerosis; Murine leukaemia virus (MLV); Myelin Basic Protein (hMBP); NC; Neuroblastoma; NIH/3T3; oncoretroviruses; PA317; Packaging cells; Peripheral artery disease; PG13; PG368; Phoenix; pol (polymerase); pro (PR); production; purification; Recurrent High-Grade Gliomas; Renal Cancer; replication-competent retroviruses (RCRs); Retroviridae family; retrovirus; reverse transcriptase (RT); Rous Sarcoma Virus (RSV) promoter; SCID-X1; simian; Simian Foamy Virus - SFV; Spleen Focus Forming Virus (SFFV) U3 sequence; streptavidin-biotin affinity; SV40; T-cell receptor specific for Wilms tumour antigen 1; Te Fly A; Te Fly Ga18; trehalose; Vascular endothelial growth factor (VEGF) Fibulin-5; vesicular stomatitis virus glycoprotein G (VSV-G); Wiskott-Aldrich- Syndrome; X-CGD
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test