Full text loading...
-
Production, Purification and Characterization of Adeno-Associated Vectors
- Source: Current Gene Therapy, Volume 10, Issue 6, Dec 2010, p. 423 - 436
-
- 01 Dec 2010
Abstract
The use of recombinant adeno-associated viral vectors (rAAVs) as gene transfer tools has increased dramatically during the past several years, establishing AAV as the vector of choice for many therapeutic applications. With the steady advance of the field toward clinical studies, and the isolation and engineering of several novel AAV serotypes, efficient, scalable, and versatile production and purification methods are continuously under development. Here, we review the current state of the art in the various production and purification methods for rAAVs. Classical parameters and methodologies to characterize rAAV stocks will be also discussed.
© Bentham Science Publishers