Skip to content
2000
Volume 27, Issue 4
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Background: An endophytic fungal strain was isolated from the seagrass and investigated to identify its antimicrobial constituents and characterize its metabolome composition. The ethyl acetate extract of this fungus exhibited antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) as well as an anti-quorum sensing effect against . Methods: The crude extract was profiled by UHPLC-HRMS/MS, and the dereplication was assisted by feature-based molecular networking. As a result, more than twenty compounds were annotated in this fungus. To rapidly identify the active compounds, the enriched extract was fractionated by semipreparative HPLC-UV applying a chromatographic gradient transfer and dry load sample introduction to maximise resolution. The collected fractions were profiled by 1H-NMR and UHPLC-HRMS. Results: The use of molecular networking-assisted UHPLC-HRMS/MS dereplication allowed preliminary identification of over 20 compounds present in the ethyl acetate extract of . The chromatographic approach significantly accelerated the isolation of the majority of compounds present in the active extract. The one-step fractionation allowed the isolation and identification of eight compounds (1-8). Conclusion: This study led to the unambiguous identification of eight known secondary metabolites as well as the determination of their antibacterial properties.

Loading

Article metrics loading...

/content/journals/cchts/10.2174/1386207326666230707110651
2024-03-01
2025-07-15
Loading full text...

Full text loading...

/content/journals/cchts/10.2174/1386207326666230707110651
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test