Electrochemiluminescence Sensor based on Electrospun Crosslinked Carbon Nanofibers for the Detection of Difenidol Hydrochloride

Background: Cross-linked porous carbon nanofibers (CNF) were successfully prepared by electrospinning and high-temperature carbonization. Polyacrylonitrile (PAN) as the carbon source and genipin as the cross-linking agent were used to prepare cross-linked porous carbon nanofibers (CNF). Materials and Methods: The field emission scanning electron microscopy (SEM), transmission electron microscope (TEM), automatic specific surface and porosity analyzer Brunner Emmet Teller (BET), X-ray diffraction (XRD), and a laser confocal microspectroscope (Raman, XploRA PLUS, Horiba) were used to characterize the materials. The CNF suspension was dropped on the surface of the bare glassy carbon electrode by the drip coating method to obtain a CNF-modified electrode. Cyclic voltammetry was used to study the electrochemiluminescence behavior of difenidol hydrochloride on CNF-modified glassy carbon electrode (Glassy Carbon Electrode, GCE). Results and Discussion: Herein, we synthesised a kind of crosslinked carbon nanofibers and designed a novel ECL biosensor. Under the optimal conditions, the concentration of difenidol hydrochloride exhibited a linear relationship with the peak current in the range of 8.0×10^-8 to 1.0×10^-4 mol/L, with the correlation coefficient of R²=0.997, and a low detection limit (1.2×10^-8 mol/L). Difenidol hydrochloride in difenidol hydrochloride tablets was tested, and the recovery rate of sample addition was estimated to be 83.17%-92.17%, and the RSD value to be <5.0%. The designed platform exhibited excellent analytical performance for difenidol hydrochloride determination.