A Fusion Method Based on Alignment Software with SNP and Indel Detection Methods

Background: With the advent of the second-generation sequencing technology, the discovery of sequence alignment and sequence variation is a long-standing challenge Methods: A method based on general alignment software, SNP, and Indel software tools was proposed in this paper. By comparing the advantages of traditional alignment software, we can produce the best alignment. Results: SNP and Indel detection tools fusion research found that different depth of sequencing effect is different. When the sequence depth reaches a certain value, one of the softwares is selected for testing Conclusion: Finally, the experimental verification shows that SNP and Indel have certain advantages in comparing the effects of the fusion method.