Optical Biosensors as a Tool for Early Determination of Absorption and Distribution Parameters of Lead Candidates and Drugs

Specific molecular interactions provide a fundamental mechanism for selectivity in every aspect of biological structure and function. The ability to measure quantitatively such interaction properties across a wide range of affinity, size, and purity is a growing need. A short review on the use of the optical biosensor techniques is presented, focused on its application for determining the absorption and distribution parameters of drugs and lead compounds. The basic biosensor technology principles are described together with some immobilization methods commonly used for the preparation of selective and specific biosensor surfaces for assays. Some relevant research topics in the field of small molecule recognition phenomena are presented as examples, including binding to plasma proteins, and binding to lipid membranes, in the frame of ADME (absorption, distribution, metabolism and excretion) parameter determinations. These applications demonstrate the applicability of such techniques to the study of low mass compounds and illustrates their potential for the screening of libraries of compounds with regard to their binding to target bio-molecules as part of drug development.