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image of Anthelmintic Activity of Carica papaya Leaf Extracts: Insights from In Vitro and In Silico Investigations

Abstract

Introduction

Helminthiasis remains a major global health concern. Exploring natural alternatives due to drug resistance and synthetic drug side effects has become increasingly urgent.

Method

This study investigates the anthelmintic potential of leaf extracts (CPLE) against , along with elucidating the underlying structural alterations and molecular interactions. underwent methanolic extraction. Gas chromatography-mass spectrometry analysis revealed 11 active phytochemical compounds within CPLE. The anthelmintic activity was evaluated against , with CPLE demonstrating efficacy comparable to albendazole. Light microscopy and scanning electron microscopy depicted structural modifications in worms exposed to CPLE, characterized by reduced size, uniform shrinkage, and increased cuticle thickness.

Result

Molecular docking studies with proteins β-tubulin and β-tubulin revealed potential binding interactions of CPLE compounds, notably Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester, and Albendazole oxide.

Conclusiَn

These findings suggest the anthelmintic efficacy of CPLE and provide insights into its mode of action at the molecular level.

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2024-10-09
2024-11-26

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/content/journals/cchts/10.2174/0113862073341577240925100048
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Anthelmintic Activity of Carica papaya Leaf Extracts: Insights from In Vitro and In Silico Investigations
Bentham Science Publishers ; https://doi.org/10.2174/0113862073341577240925100048
/content/journals/cchts/10.2174/0113862073341577240925100048
/content/journals/cchts/10.2174/0113862073341577240925100048
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  • Article Type:     Research Article
Keywords: structural alterations ; Helminthiasis ; molecular docking ; earthworms ; Carica papaya extract

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