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image of In-vitro and In-silico α-amylase Inhibition Activity of Carlina Oxide and Aplotaxene Isolated From the Roots of Carthamus caeruleus and Rhaponticum acaule

Abstract

Background

Numerous natural products have been successfully developed for clinical use in the treatment of human diseases in almost every therapeutic area.

Objectives

This work aimed to assess the and α-amylase inhibition activities of carlina oxide and aplotaxene, isolated from the roots of and respectively.

Methods

The essential oil from roots was obtained using a Clevenger-type apparatus, and the hexanoic extract from the roots of was obtained through maceration. Major components of each plant were separated column chromatography. The α-amylase inhibition activity was evaluated using porcine pancreatic α-amylase, while the molecular docking study was conducted using the Molecular Operating Environment (MOE) with three types of α-amylase: human salivary, pancreatic α-amylase and α-amylase (PDB: 1Q4N, 5EMY, 7P4W respectively).

Results

The α-amylase inhibition results for the essential oil, the hexanoic extract, carlina oxide and aplotaxene showed that carlina oxide exhibited significant activity with IC of 0.42 mg/mL. However, the study showed no interaction between aplotaxene and the three α-amylase enzymes, whereas carlina oxide demonstrated one pi-cation interaction with 5EMY with the amino acid TYR 62 at a distance of 4.70 Å and two pi-H interactions with 7P4W with the amino acid LYS 383 at distances of 4.31 and 4 .03 Å.

Conclusion

In conclusion, carlina oxide has the potential to serve as an alternative agent for α-amylase inhibition, contributing to the reduction of postprandial hyperglycemia.

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2024-11-22

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In-vitro and In-silico α-amylase Inhibition Activity of Carlina Oxide and Aplotaxene Isolated From the Roots of Carthamus caeruleus and Rhaponticum acaule
Bentham Science Publishers ; https://doi.org/10.2174/012212796831732824091804122
/content/journals/ccb/10.2174/012212796831732824091804122
/content/journals/ccb/10.2174/012212796831732824091804122
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  • Article Type:     Research Article
Keywords: α-amylase inhibition ; molecular docking ; Carthamus caeruleus ; aplotaxene ; carlina oxide ; Rhaponticum acaule ; postprandial hyperglycemia

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