Effect of Ginger Extract on 4T1 Breast Cancer Cell Line in Balb/c Mouse

Background: By considering [6]-gingerol as an important polyphenol, ginger has been related to cancer. Objective: The main objective of this study is to evaluate the ginger extract on the expressions of four genes from different categories involved in breast cancer in Balb/C mice after 14 days. Methods: In terms of this research, ethanol extraction methods for extracting ginger rhizomes with the goal of increasing [6]-gingerol were used. In this regard, a MTT test was accomplished for the whole ginger extract to confirm its effect on the 4T1 breast cancer cell line (murine mammary carcinoma cell). Afterward, the transplanted breast cancer Balb/C mouse was used to be treated with the whole ginger extract (0.02 g/kg of mouse weight). The expressions of STAT3, β-catenin, P53, APC, genes were evaluated, after 14-day of treatment. Results: The results showed that 70% ethanol and microwave extraction method can be considered as the best method for extracting ginger extract. The expression of β-catenin has decreased in the blood and liver of the treated group. Moreover, the expression of STAT3 oncogenes has significantly decreased in the liver tissue. In addition, the expressions of APC suppressor genes have increased in the blood and liver. Conclusion: In conclusion, treatment by 0.02 g/kg of mouse weight of ginger extract after 14 days increased the expressions of APC as suppressor genes and a β-catenin oncogene in blood and liver of the breast cancer group.