Current Biotechnology - Volume 9, Issue 2, 2020

The diversity of indole concerning its production and functional role has increased in both prokaryotic and eukaryotic systems. The bacterial species produce indole and use it as a signaling molecule at interspecies, intraspecies, and even at an interkingdom level for controlling the capability of drug resistance, level of virulence, and biofilm formation. Numerous indole derivatives have been found to play an important role in the Read More

Introduction: Selinum vaginatum is an endemic Indian plant present on high altitude and is known as “Bhootkeshi,” by the locals. The root of plant Selinum vaginatum commonly known as “Bhootkeshi” from the family Umbellifercae is an aromatic herbal drug. Selinum vaginatum has been used traditionally for curing seizures, epilepsy, mental illness, and hysteria. This plant has shown therapeutic effects as used by the locals. The Read More

Background: The focus of this study was to prepare and characterize the single-chain variable fragment antibody (scFv)-coupled immunoaffinity column for purification of subtilisin BRC. Methods: The scFv against subtilisin BRC was immobilized onto CNBr-activated Sepharose 4B. Adsorption isotherm for subtilisin BRC on scFv-BRC-coupled Sepharose 4B was obtained and calculated the maximum binding capacity. The extracti Read More

Background: Due to the last two decades of drought disaster, which resulted in the loss of the main part of Urmia Lake water and changed the natural conditions of an environment, especially ionic strength. Objective: We aimed to isolate and characterize halophilic and halotolerant bacteria in Urmia Lake, Iran, 2015. Urmia Lake is a permanent and salty inland lake located in the Azerbaijan region in northwestern Iran. Method Read More

Background: Centella asiatica (L.) is an herbaceous, frost-tender perennial plant belonging to the Apiaceae family. It is rich in important secondary metabolites such as terpenoids, flavonoids and saponins. It is used as a traditional medicine for skin infection, cough and asthma. Methods: The present study was intended at the quantitative and qualitative analysis of its secondary metabolites. Thin Layer Chromatography (TLC) Read More

Background: Azo dyes are widely used recalcitrant chemicals and may promote environmental hazards. Amongst the known azo dyes is CI Direct Red 28 (C32H22N6Na2O6S2) aka Congo Red (CR), which is a known mutagen and carcinogen. In this regard, this work aimed at the biodegradation of a CR-containing synthetic effluent, and the evaluation of the ecotoxicity of post-treatment residues. Methods: Lentinus sp. Laccase (La Read More

Background: Proteases play a crucial role in the pharmacological properties of latex producing plants. Some of them exhibited intervention with fibrinogenolysis and/or fibrinolysis, two crucial wound healing events. Objective: To evaluate wound healing potential of crude and partially purified enzyme from Tabernaemontana divaricata (stem and latex). Materials and Methods: Proteolytic activity, clot inducing/dissolving potentia Read More

Background: Polyhydroxyalkanoates (PHA) are polyesters produced by various microorganisms. The major drawback of PHA, which is restricting its large-scale production in industries, is its high cost of raw materials and lower PHA concentration. Objective: The present study aimed to enhance the production of PHA from Wickerhamomyces anomalus VIT-NN01 by optimizing various nutrient and environmental stress conditions a Read More