Current Biotechnology - Volume 8, Issue 1, 2019

Background: Laccases are important enzymes that have numerous applications in different biotechnological sectors. Objective: The aim was to purify laccase from Aspergillus flavus PUF5, successfully immobilize it on coconut fiber and characterize different physical and kinetic properties under both free and immobilize conditions. Methods: Laccase from A. flavus PUF5 was purified using ammonium sulfate precipitation, fo Read More

Background: O-phospho-L-serine sulfhydrylase from the hyperthermophilic archaeon Aeropyrum pernix K1 (ApOPSS) is thermostable and tolerant to organic solvents. It can produce nonnatural amino acids in addition to L-cysteine. Objective: We aimed to obtain higher amounts of ApOPSS compared to those reported with previous methods for the convenience of research and for industrial production of L-cysteine and non- Read More

Background: The focus of this study was the selection of a single chain variable fragment antibody (scFv) against subtilisin BRC, a fibrinolytic enzyme using phage display, and to characterize the interaction between the antibody and subtilisin BRC. Methods: The subtilisin BRC-specific phage clones were selected using Griffin.1 scFv phage library and sequenced. The gene of subtilisin BRC-specific scFv (scFv-BRC) from s Read More

Background: Musa paradisiaca (Banana plant), which belongs to the family of Musaceae, is a well-known herbaceous flowering edible plant. The flower, fruit, and stem part of the plant have been used for nutrients and health benefits. Objective: The aim of this study is to determine the secondary metabolites, proximate composition, minerals, heavy metals, and anti-oxidant activity of three edible parts (flower, unripe fru Read More

Background: Plant in vitro culture systems serve as a useful tool to study the regulatory routes which are related to plant growth and survival under altered environmental conditions. Methods: Callus culture of Suaeda monoica and Suaeda nudiflora were established for studying the salt tolerance mechanism at the cellular level. Calli of both the species were induced from seedling’s epicotyls on Murashige and Skoog (MS) medium s Read More

Background: The two wild plants viz. Sphenoclea zeylanica and Sphaerantus peguensis are seasonally consumed as vegetables by the Bodo people in Assam of North East India. Wild vegetables are considered as one of the cheapest sources for human nutrition that contains rich sources of numerous minerals and bioactive compounds which on consumption can contribute several health benefits against various diseases. Obj Read More