Current Biotechnology - Volume 7, Issue 5, 2018

Background: Quercetin and apigenin are a new class of drug targets for Helicobacter pylori D-alanine- D-alanine ligase (HpDdl). However, their clinical use is limited by its oral bioavailability and hence requires its molecular modification. The present study is an attempt to investigate the insights of competitive inhibition of these compounds. Methods: In silico ligand-protein interaction was carried out, predicting molecular Read More

Objectives: Extraction and manipulation of bacterial nucleic acid directly from soil has emerged as indispensable technique to explore the genomic potential of uncultured microbes. Major difficulty in separating humic acid from soil DNA is attributed to its strong interactions with the humic acid. Previously several methods were used to purify DNA, however the kits and material used during purification is costly and lack efficieny al Read More

Background: Tweaking CHO cell metabolism in fed-batch processes to provide sufficient cellular energy for the synthesis of recombinant proteins at high specific rates forms the basis for achieving superior process yields. Despite the growing understanding of the backgrounds of cellular phenotypes, like the Warburg effect, there is still an unmet need to translate theory into practice. Objective: This study presents a new c Read More

Objectives: The main objective of this study was to investigate the molecular diversity of α- subunit of bacterial Ring Hydroxylating Dioxygenases (RHD) in agriculture soil. These enzymes catalyze break down of polyaromatic hydrocarbon to non-toxic form without forming harmful products like 3-PBA (3-Phenoxybenzoic acid). To meet our objectives, we carried out PCR amplification, cloning and analysis of genes encoding partial Read More

Background: Dextran is a bacterial polysaccharide composed of chains of D-glucose units. Most of the commercial strains for dextran production belong to Leuconostoc Spp. Dextran has many uses in the pharmaceutical, food and textile industries. Objectives: The objective of this study was to elucidate the characteristics and structural aspect of the dextran synthesized by purified novel dextransucrase of Acetobacter tropicalis. Read More

Objective: The objective of this study was to analyze microbial quality of raw milk and some milk products from Nanded region and to determine their antibiotic susceptibility pattern. Method: A total of 80 samples was collected and screened for the presence of some bacterial pathogens. These bacterial pathogens were identified based on morphological characters and biochemical tests and were further subjected for anti Read More

Introduction: Scientists and the public are interested in rapid and continuous monitoring of pesticide residues present in or on food using sensitive and low-cost tools. The present study developed and optimized a sensitive visual screening tool using a bio-based paper for the detection of cholinesteraseinhibiting insecticides. Method: The technique based on the change of the intensity of a purple color formed from diazoniu Read More