Current Biotechnology - Volume 6, Issue 4, 2017

Background: Microorganisms such as filamentous fungi are important sources of enzymes and secondary metabolites with wide application in biotechnological processes conducted in different industrial areas. Aspergillus, one of the most important genera of filamentous fungi for this purpose, has been used as a model to understand different biological processes. Despite the existence of some pathogenic Aspergillus s Read More

Background: Current studies about lipases production using agro-industrial residues and newly isolated microorganisms aimed at increasing economic attractiveness. Objective: The present study evaluated the lipase production from Aspergillus niger using canola cake as substrate and its extraction and characterization. Methods: Firstly, the microorganism was identified using sequencer ABI PRISM 3100 Genetic Analyzer. A Read More

Background: Lipases are enzymes widely used in many industrial applications. However, its use can be increased by reducing costs of production, being important to this effort the screening of new microorganisms producing and the use of low cost culture mediums, such as agricultural residuals. Objective: To produce lipases using two new isolates of filamentous fungi in solid-state fermentation. Methods: The selection of Read More

Backgrond: The aqueous two-phase systems (ATPS) have been used as the first purification step in enzyme industrial isolation. Such systems allow the removal of large amounts and different types of contaminants by a simple and economic unit operation. Objective: The tannase extraction was conducted through ATPS composed of polyethylene glycol (PEG) and sodium citrate salt (PEG/citrate). Method: A factorial design model ( Read More

Background: Manganese peroxidase (MnP) is a common extracellular ligninolytic peroxidase produced by Pleurotus spp. It catalyzes the H2O2-dependent oxidation of Mn2+ into highly reactive Mn3+, which in turn oxidizes phenolic and nonphenolic compounds including a large list of xenobiotics such as synthetic dyes. Objective: To purify and characterize the MnP of Pleurotus pulmonarius and to evaluate its capability Read More

Background: Endophytic fungi are considered a source of novel activities, compounds and biotechnological processes with great and underexplored potential. The aims of this paper were: a) to evaluate the antibiotic activity of mangrove endophytic fungi against the human pathogens Staphylococcus aureus, Salmonela typhi and Escherichia coli and against the phytopathogen Xantomonas axonopodis citri; b) to elucidat Read More

Background: This review is intended to give an account of the knowledge about enzymatic reactions performed by brazilian marine-derived fungi. These biocatalysts can offer a range of enzymes capable to produce a large spectra of added value compounds. Screenings with varied species of marine-derived fungi presented active enzymes capable of performing the biotransformation of nitriles and epoxides, bioreduction of ca Read More

Background: Plants live in a dynamic environment subjected to frequent pathogenic and environmental stresses and have evolved diverse self-defense pathways for their survival. Many of these defense responses are energy-intensive and antagonistic against each other to enable prioritization of strong targeted responses. Viruses cause significant damage in the agricultural and natural world. They are obligate intr Read More

Background: Biodegradation of lignocellulosic materials to monomeric sugars by the action of cellulolytic enzymes has recorded a significant value for industrial processes. These sugars can be converted to ethanol which is of great economic value to salvage the ever-increasing global dependence on fossil fuel and its gradual depletion. Exploitation of this cellulosic groundnut shell biomass possesses both significant indu Read More

Background: β-Glucosidases are terminal enzymes in cellulose hydrlysis which hydrolyze glycosidic bonds between two or more carbohydrates. Not much information is available on purification and characterization of β-glucosidase from Penicillium janthinellum. Here we report the purification and characterization of thermostable β-glucosidase from P. janthinellum mutant EU2D-21 produced under submerged conditions. Metho Read More