Current Biotechnology - Volume 11, Issue 1, 2022

Objectives: Fatty acid alkyl esters (FAAEs) are a family of natural neutral lipids and can be produced cleanly and sustainably by esterification of free fatty acids (FFAs) with short chain alcohol using enzymatic catalysts. In this study, the use of lipases was evaluated in enzymatic esterification of oleic acid with ethanol using the combination of biocatalysis and sonochemistry (ultrasound) in the absence of co-solvents. Methods: Rea Read More

The α-amylase is one of the most promising commercial enzymes with tremendous applications in various industries. Microbial α-amylase shares almost 25-30% of the enzyme market due to its catalytic function in several industries, including sugar, detergent, paper, textile, pharmaceutical industries, etc. The α-amylase hydrolyzes glycosidic linkages of structural components of starch, resulting in maltose, glucose, and high Read More

Lupus is a heterogeneous and inflammatory autoimmune disease that affects the immune system of the body. This disease affects multiple tissues and organs. The most frequent kind of lupus is systemic lupus erythematosus (SLE). SLE is an autoimmune illness in which the immune system targets the body's tissues, resulting in extensive inflammation and tissue destruction in the organs involved. Joints, skin, brain, lungs, kid Read More

Background: Salmonella serovars contamination of food items shows a negative effect on the food industry and causes a great hazard for the consumers. Although developed hygiene methods, Salmonella serovars are still a high risk to human health, and new methods for the identification of Salmonella spp. are needed. Objective: Bacteriophages have been considered a good method for detecting bacteria owing to their hi Read More

Background: Polyhydroxyalkanoates (PHAs) are biodegradable, biocompatible, and non-toxic polymers synthesized by bacteria that may be used to displace some petroleum-based plastic materials. One of the major barriers to the commercialization of PHA biosynthesis is the high cost of production. Objective: Oxygen-limitation is known to greatly influence bacterial cell growth and PHA production. In this study, the growth Read More

Background: Diabetes mellitus is a disease and endocrine disorder. It is a growing health problem in various countries. The prevalence of diabetes is rising worldwide; around 5.4% rise in patients with diabetes is expected by 2025. The World Health Organization (WHO) has reported that 173 million adults are suffering from diabetes mellitus in developing counties. In this research, estimation of glucose levels was performed in Read More

Background: The most widely used thermostable enzymes are the amylases in the starch industry. These are among the most important enzymes and are of great significance in present day biotechnology. Objective: The main objective of the present study was to enhance α-amylase production from Bacillus licheniformis using Response Surface Methodology (RSM) and the purification of the enzyme to homogeneity. M Read More

Background: Retinoblastoma, a malignancy occurring in the juvenile cells of the retina, is responsible for light detection. It is one of the most emerging ra re childhood and infant cancer. It is initiated by the mutation in Rb1, a first tumor suppressor gene located on chromosome 13q14. Rb1 protein is responsible for cell cycle regulation. Methods: In our study, secondary and 3D-Structural predictions of Rb1WT and Rb1R6 Read More

Background: Essential oils (EOs) are a mixture of volatile compounds of plant origin, which possess substantial biological activities such as antioxidant, antimicrobial, and antifungal activity. Objective: This study aimed to determine the chemical composition, antioxidant, and antibacterial activity of essential oil isolated from Cymbopogon winterianus Jowitt. Methods: The hydro-distillation method was used for the isolation o Read More