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Volume 13, Issue 4
  • ISSN: 2211-5501
  • E-ISSN: 2211-551X

Abstract

Introduction

The waste-to-wealth concept was applied in this study. Our main objective was to identify and evaluate several microorganisms responsible for the production of important industrial enzymes using sludge from palm oil processing. We were able to isolate several bacteria that are tyrosinase producers: . Laccase producers and , as well as lipase producers.

Methods

In addition, we focused specifically on because we knew that it produces tyrosinase. Lipase is another target enzyme, and was found to be a hyperproducer. Production conditions included a 24-hour incubation period at 40°C and a pH of 6.0, while typical substrates such as starch and coconut oil were used.

Results

Preliminary protein purification studies identified a 43 kDa lipase that is active at pH 7.0 and 40°C. Salts such as NaCl, various detergents such as Triton X-100 and Tween-80 as well as many metal ions enhanced the activity and made the enzyme unique in its biological function.

Conclusion

Even with EDTA (2.5 mM), which does not completely block its function, only 40% inhibition was observed. Only a few organic solvents such as butanol, acetone and DMF are involved in inhibiting its activity.

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2024-10-08
2025-01-19

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Characterization of a Polyphenol Oxidase and Lipase Produced by Microbes Derived from Palm Oil Sludge
Curr. Biotechnol. 13, 223 (2024); https://doi.org/10.2174/0122115501337787240930180432
/content/journals/cbiot/10.2174/0122115501337787240930180432
/content/journals/cbiot/10.2174/0122115501337787240930180432
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  • Article Type:     Research Article
Keyword(s): bioremediation, enzymes, lignin; palm oil sludge; Polyphenol oxidases; tyrosinase

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