Effect of Various Parameters and Mechanism of Reversal Order of Elution in Chiral HPLC

Background: Chiral separation involves many phenomena in which the elution order of the enantiomers has its unique position. The phenomenon of elution order of the enantiomers has also been used in the determination of optical purity which is favorable to elute the major component after minor enantiomeric impurity but the main problem is that, this phenomenon is rare. Results: This review rumors the reversal order of elution of many chiral molecules in HPLC. Besides, this review pronounces the effects of pH, derivatisation of drugs, the composition of the mobile phase, and temperature on the reversal order of elution of chiral drugs. The efforts are also made to discuss the possible future perspectives of reversal order of elution. Conclusion: Various parameters such as pH, mobile phase composition, temperature, and chemical structure of the analytes play a role in the phenomena of the reversal order of elution of many chiral molecules which are discussed in the article.