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Stilbenes are naturally occurring plant polyphenols, which have received considerable attention due to their various biological activities, structural complexity and diversity. Rapid analysis and separation of stilbenes have been a challenge for phytochemists and analytical chemists. In recent years, major advances have been achieved by application of HPLC/MS, MS/MS and CCC. Also, many novel methods have been successfully developed. This review attempts to summarize the progress in this research area.