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image of Characterization of the Chemical Components of Pedicularis kansuensis Maxim Using Ultra-high-performance Liquid Chromatography Coupled to Quadrupole‐Time‐of‐Flight Tandem Mass Spectrometry

Abstract

Background

Maxim. is a commonly used Tibetan medicine that has been used for a long time to clear heat and detoxify, remove dampness and diuresis, treat sores, and nourish. It has favorable biological activities such as anti-inflammatory, antioxidant, and anti-fatigue. However, to date, only a handful of studies have utilized traditional separation and purification methods to investigate the chemical constituents of Maxim. This scarcity of comprehensive and systematic research on its chemical composition impedes the further exploration of its biological activity and clinical applications.

Objective

It is imperative to develop an effective, dependable, and expeditious methodology for the systematic analysis and comprehensive characterization of the diverse chemical constituents found in Maxim through cracking pathways and patterns.

Methods

This study used negative ion mode UHPLC-QTOF-MS/MS and 8 reference standards to comprehensively investigate the collision-induced dissociation tandem mass spectrometry (CID-MS/MS) fragmentation pathway of chemical components in Maxim. A method based on ultra-high performance liquid chromatography and quadrupole time-of-flight mass spectrometry combined with data analysis software was established for screening and identifying targeted and non-targeted components of Maxim.

Results

A total of 150 compounds were identified in Maxim, including 43 acylglucoside, 36 iridoid glycosides, 19 lignans, 41 flavonoids, and 11 other compounds.

Conclusion

This research has meticulously crafted an efficient and comprehensive methodology for the detection of intricate compounds. It has been successfully implemented in the analysis and identification of chemical constituents within Maxim, thereby laying a solid foundation for further in-depth exploration of this species. Moreover, this work serves as a valuable reference for the study of other traditional Chinese medicinal herbs.

© 2025 Bentham Science Publishers
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2025-03-06
2025-04-02

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/content/journals/cac/10.2174/0115734110346855241225104655
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Characterization of the Chemical Components of Pedicularis kansuensis Maxim Using Ultra-high-performance Liquid Chromatography Coupled to Quadrupole‐Time‐of‐Flight Tandem Mass Spectrometry
Bentham Science Publishers ; https://doi.org/10.2174/0115734110346855241225104655
/content/journals/cac/10.2174/0115734110346855241225104655
/content/journals/cac/10.2174/0115734110346855241225104655
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  • Article Type:     Research Article
Keywords: tibetan medicine ; UHPLC-QTOF-MS/MS ; Chemical profiling, Pedicularis kansuensis Maxim

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