Cd2+ Mediated AuNCs for Simultaneous Detection of L-cysteine and Homocysteine

Huiru Zheng; Yi Xiao; Shengda Qi; Honglin Zhai

doi:10.2174/0115734110346349241208043105

image of Cd2+ Mediated AuNCs for Simultaneous Detection of L-cysteine and Homocysteine

Cd2+ Mediated AuNCs for Simultaneous Detection of L-cysteine and Homocysteine
Authors: Huiru Zheng¹, Yi Xiao¹, Shengda Qi¹ and Honglin Zhai¹
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¹ College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730030, China
Source: Current Analytical Chemistry
Available online: 15 January 2025
DOI: https://doi.org/10.2174/0115734110346349241208043105
- Received: 20 Aug 2024
- Accepted: 18 Nov 2024
- Available online: 15 Jan 2025

Abstract

Background

L-cysteine (L-Cys) and homocysteine (Hcy) are the most representative of biothiol, which exist widely in organisms. L-Cys is one of the essential amino acids, which can be absorbed from protein-rich food and plays a considerable role in various physiological processes. Hcy is a vital intermediate in normal mammalian metabolism of methionine but does not occur in the diet. Therefore, it is significant to exploit a rapid and sensitive strategy to measure L-Cys and Hcy.

Methods

Herein, we designed an “on-on” fluorescent platform for detecting L-Cys and Hcy with gold nanoclusters (AuNCs) as probes. During the sensing process, cadmium ions (Cd²⁺) acted as mediating substances to connected AuNCs and L-Cys (or Hcy), and triggered aggregation-induced emission (AIE) effect.

Results

The linear ranges achieved with fluorimetry of L-Cys and Hcy were 0.1-10.0 μM and 0.1-20.0 μM, respectively. Moreover, this fluorescent probe was successfully used to determine the L-Cys concentration in actual samples, and showed excellent recovery.

Conclusion

Furthermore, the mechanism for sensing L-Cys and Hcy has been exhaustively investigated.

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