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In recent years, microfluidics systems have emerged as powerful tools for biological analysis, integrating entire analytics protocols into a single chip platform. This article aims to review recent developments in microfluidics systems for diagnostics applications, focusing on genes, proteins, and cells. By categorizing fluids- manipulating mechanisms and biological detection approaches, the articles provide an in-depth discussion of microfluidics-based diagnostics systems, including materials and manufacturing techniques. The integration of microfluidics systems with diagnostics shows promise for the development of practical point- of-care devices. The identification and monitoring of Variants of Concern (VOCs) and Variants of Interest (VOIs) by WHO have significant implications for diagnostics, public health measures, and vaccine development. Continuous, sequencing and adaptation of diagnostic tests are essential for managing the impact of variants on diagnostics and public health measures while advancing vaccine research and development.
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