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2000
Volume 23, Issue 13
  • ISSN: 1871-5206
  • E-ISSN: 1875-5992

Abstract

Background: -coumaric acid (p-CA) is a kind of phenylpropionic acid derived from aromatic amino acids, which is widely distributed in many plants and human diets. It has strong pharmacological and inhibitory effects on a variety of tumors. However, the role of -CA in osteosarcoma, a tumor with a poor prognosis, is still unknown. Therefore, we aimed to evaluate the effect of -CA on osteosarcoma and explore its potential mechanism. Objective: This study aimed to investigate whether -CA has an inhibitory effect on the growth of osteosarcoma cells and explore its potential mechanism. Methods: MTT assay and clonogenic assay were used to detect the effect of -CA on the proliferation of osteosarcoma cells. The effect of -CA on apoptosis of osteosarcoma cells was detected by the Hoechst staining and flow cytometry. The effects of -CA on the migration and invasion of osteosarcoma cells were detected by scratch healing assay and Transwell invasion assay. Western blot and PI3K/Akt pathway activator 740Y-P were used to detect the anti-tumor mechanism of -CA on osteosarcoma cells. The effect of -CA on osteosarcoma cells in vivo was verified by an orthotopic osteosarcoma tumor animal model in nude mice. Results: MTT assay and clonogenic assay showed that -CA inhibited the proliferation of osteosarcoma cells. Hoechst stain and flow cytometry showed that -CA could induce apoptosis of osteosarcoma cells and lead to G2 phase arrest of osteosarcoma cells. Transwell assay and scratch healing assay found that -CA could inhibit the migration and invasion of osteosarcoma cells. Western blot showed that -CA could inhibit the activity of the PI3K/Akt signaling pathway in osteosarcoma cells, and 740Y-P could reverse its inhibitory effect. In vivo mouse models, -CA has an antitumor effect on osteosarcoma cells in vivo, and at the same time, it has less toxic side effects on mice. Conclusion: This study demonstrated that -CA could effectively inhibit the proliferation, migration and invasion of osteosarcoma cells and promote apoptosis. -CA may play an anti-osteosarcoma role by inhibiting PI3K/Akt signaling pathway.

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/content/journals/acamc/10.2174/1871520623666230420092439
2023-08-01
2025-03-15
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  • Article Type:
    Research Article
Keyword(s): anti-tumor; clonogenic assay; osteosarcoma; p-coumaric acid; phenylpropionic acid; PI3K/AKT
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