Skip to content
2000
Volume 19, Issue 2
  • ISSN: 1871-5206
  • E-ISSN: 1875-5992

Abstract

Background: Identification of events leading to hepatocellular carcinoma (HCC) progression is essential for understanding its pathophysiology. The aims of this study are to identify and characterize differentially expressed proteins in serum of HCC-bearing rats and the corresponding controls during cancer initiation, progression and tumorigenesis. Methods: Chemical carcinogens, N-Nitrosodiethylamine and 2-aminoacetylfluorine are administered to induce HCC to male Wistar rats. The 2D-Electrophoresis and PD-Quest analyses are performed to identify several differentially expressed proteins in serum of HCC-bearing animals. These proteins are further characterized by MALDI-TOF-MS/MS analyses. Using pathwaylinker a HCC-specific network is analyzed among the MALDITOF- MS/MS characterized proteins and their interactors. Results: Carcinogen administration caused inflammation leading to liver injury and HCC development. Liver inflammation was confirmed by increase in the levels of TNF-α and IL-6 in carcinogen treated rats. We report significant increase in expression of two differentially expressed proteins, namely, A-Raf and Fatty Acid 2- Hydroxylase (FA2H), at early stage of HCC initiation, during its progression and at tumor stage. Real-time PCR analysis of mRNA for these proteins confirmed up-regulation of their transcripts. Further, we validated our experimental data with sera of clinically confirmed liver cancer patients. Conclusion: The study suggests that FA2H and A-Raf play a major role in the progression of HCC.

Loading

Article metrics loading...

/content/journals/acamc/10.2174/1871520618666181015142810
2019-01-01
2025-05-03
Loading full text...

Full text loading...

/content/journals/acamc/10.2174/1871520618666181015142810
Loading

  • Article Type:
    Research Article
Keyword(s): Cancer; HCC-specific network; initiation; pathwaylinker; tumors; western blot
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test