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Background: The use of nanobiomaterials is increasing each day. Among the immense variety of nanomaterials developed and studied the hydroxyapatite is one of the most ones. Objective: In this study we developed and tested nano-hydroxyapatite dopped with Ho-166 for bone cancer. Results: The results showed that the nano-hydroxyapatite dopped with Ho-166 has a great affinity for the bone. Conclusion: The pre-clinical studies support the use as a nano-radiopharmaceuticals for bone cancer treatment and diagnosis.