Genome Editing in Cyanobacteria
- Authors: Bathula Srinivas1, Prakash M. Halami2
-
View Affiliations Hide AffiliationsAffiliations: 1 Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam 517426, India 2 Department of Microbiology and Fermentation Technology, CSIR-Central Food Technological Research Institute, Mysuru-570020, India
- Source: Genome Editing in Bacteria (Part 2) , pp 262-277
- Publication Date: April 2024
- Language: English
Genome Editing in Cyanobacteria, Page 1 of 1
< Previous page | Next page > /docserver/preview/fulltext/9789815223798/chapter-9-1.gifCyanobacteria are potential organisms being exploited for a wide range of biotechnological applications. They are photosynthetic bacteria and grow in a carbonfree medium and become attractive hosts for biotechnology industries. Cyanobacteria can utilize solar energy and atmospheric CO2 for the growth and synthesis of biomolecules. It is used in many large-scale preparations of various bioproducts such as pharmaceuticals, biofuels, etc. Cyanobacteria become target organisms for the next generation of biofactories for producing desired products with a low-cost technology. The problem in the metabolic engineering of Cyanobacteria is due to ploidy. It has multiple copies of chromosomes ranging from 3-218 copies. There are 12 copies of the genome in Synechocystis PCC 6803 and 3 copies in Synechococcus PCC 7942. Segregation analysis in the conventional genetic approaches of Cyanobacteria becomes laborious due to its polyploidy. Modern genome editing tools such as CRISPR-Cas9 and 12 are available to perform genome editing. CRISPR-Cas9 has been used in a wide range of Cyanobacteria such as Synechococcus elongates UTEX 2973, Synechocystis sp. PCC 6803. To avoid toxic effects caused by Cas-9, a low-level expression system is adopted in Cyanobacteria. Cas-9 base genome editing was applied in Synechococcus and produced succinate 11-fold higher than the normal. Cas-9 is used to cure plasmids in Synechocystis sp. PCC 6803 to develop a shuttle vector for heterologous expression. Another variant of genome editing tool is CRISPR-Cas12a, which is successfully used in Synechocystis sp.
-
From This Site
/content/books/9789815223798.chapter-9dcterms_subject,pub_keyword-contentType:Journal105