Skip to content
2000

Genome Editing in Streptomyces

image of Genome Editing in Streptomyces

Streptomyces are Gram-positive, filamentous bacteria belonging to the group actinomycetes. This bacterium is important to the modern industrial world because of the presence of 20-50 biosynthetic gene clusters (BGCs). BGCs contain the genes for the production of industrially important natural products (NP), which includes antibiotics, anti-tumor drugs, anti-depressants, etc., naturally originated from this microorganism. Strain improvement is required to enhance the production of these NP in Streptomyces. Different methods have been used to enhance NP production and strain improvement. In this chapter, we will be discussing strain improvement of Streptomyces species by different genome editing tools. The information, which is put together, includes the basic techniques used for genome editing to the most advanced CRISPR/Cas system associated genome editing in Streptomyces (PCR targeting system, Cre-loxP recombination system, I SceI meganuclease promoted recombination system and CRISPR/Cas system). The authors have discussed about multiplex automated genome editing (MAGE) tool associated with CRISPR/Cas system.

/content/books/9789815223798.chapter-10
dcterms_subject,pub_keyword
-contentType:Journal
10
5
Chapter
content/books/9789815223798
Book
false
en
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test