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2000
Volume 19, Issue 5
  • ISSN: 1574-888X
  • E-ISSN: 2212-3946

Abstract

Background: To investigate the roles of extracellular vesicles (EVs) secreted from bone marrow mesenchymal stem cells (BMSCs) and (highly expressed in BMSC EVs) in hepatic ischemia128;’ reperfusion injury (HIRI). Approaches and Results: We constructed a HIRI mouse model and pretreated it with an injection of agomir-, agomir-NC, BMSC-EVs or control normal PBS into the abdominal cavity. Compared with the HIRI group, HIRI mice preinjected with BMSC-EVs had significantly decreased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and alleviated liver necrosis (P<0.05). However, compared with HIRI+NC mice, HIRI+ mice had significantly increased ALT and AST levels, aggravated liver necrosis, and increased apoptosis-related protein expression (P<0.05). The proliferation and apoptosis of AML-12 cells transfected with were significantly higher than the proliferation and apoptosis of AML-12 cells in the mimic NC group (P<0.01) after hypoxia induction. SMAD4 was proven to be a target gene. Furthermore, compared to HIRI+NC mice, HIRI+ mice displayed extremely reduced SMAD4 expression and increased levels of wnt1, β-catenin, c-Myc, and Cyclin D1. Conclusion: Our findings reveal the role and mechanism of in HIRI and provide novel insights for the prevention and treatment of HIRI; for example, EVs derived from BMSCs transfected with might demonstrate better protection against HIRI.

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/content/journals/cscr/10.2174/1574888X19666230901140628
2024-07-01
2025-02-06
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  • Article Type:
    Research Article
Keyword(s): apoptosis; BMSC-EVs; MiR-27b-3p; SMAD4; Wnt/β-catenin
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