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Light can act as an effective and strong agent for the cross-linking of biomaterials and tissues and is recognized as a safe substitute for chemical cross-linkers to modify mechanical and physical properties and promote biocompatibility. This review focuses on the research about crosslinked biomaterials with different radiation sources such as Laser or ultraviolet (UV) that can be applied as scaffolds, controlled release systems,and tissue adhesives for cornea healing and tissue regeneration.