Skip to content
2000
Volume 15, Issue 5
  • ISSN: 1566-5232
  • E-ISSN: 1875-5631

Abstract

Folic acid (FA) has high affinity to folate receptors (FRs), which have three isoforms: FRα, FRβ and FRγ. Among them, FRα is a tumor specific receptor, as it is frequently over-expressed in diverse malignancies but not in normal tissues. In this study, we have conjugated FA to a chitosan-poly(ethylenimine) copolymer, and have confirmed the low cytotoxicity of the product (namely “CP1.3K-FA”) in cancer cells. The transfection efficiency of CP1.3K-FA has been shown by the EGFP transfection assay to be higher than that of the unmodified chitosan-poly(ethylenimine) copolymer under optimal conditions. Results of the luciferase activity assay have also indicated that the transfection efficiency of CP1.3K-FA is comparable to that of Fugene HD in B16 and U87 cells. Our results have suggested that CP1.3K-FA warrants further development as a vector for gene delivery in cancer cells.

Loading

Article metrics loading...

/content/journals/cgt/10.2174/1566523215666150812120347
2015-10-01
2025-07-07
Loading full text...

Full text loading...

/content/journals/cgt/10.2174/1566523215666150812120347
Loading

  • Article Type:
    Research Article
Keyword(s): Chitosan; Folic acid; Gene transfer; Non-viral vectors; Poly(ethylenimine); Targeting
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test