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Production of Non Viral DNA Vectors
- Source: Current Gene Therapy, Volume 10, Issue 6, Dec 2010, p. 487 - 507
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- 01 Dec 2010
Abstract
After some decades of research, development and first clinical approaches to use DNA vectors in gene therapy, cell therapy and DNA vaccination, the requirements for the pharmaceutical manufacturing of gene vectors has improved significantly step by step. Even the expression level and specificity of non viral DNA vectors were significantly modified and followed the success of viral vectors. The strict separation of “viral” and “non-viral” gene transfer are historic borders for scientists and we will show that both fields together are able to allow the next step towards successful prevention and therapy. Here we summarize the features of producing and modifying these non-viral gene vectors to ensure the required quality to modify cells and to treat human and animals.