Indirect Determination of Amikacin by Gold Nanoparticles as Redox Probe

Background: Amikacin is an aminoglycoside antibiotic used for many gram-negative bacterial infections like infections in the urinary tract, infections in brain, lungs and abdomen. Electrochemical determination of amikacin is a challenge in electroanalysis because it shows no voltammetric peak at the surface of bare electrodes. Objective: In this approach, a very simple and easy method for indirect voltammetric determination of amikacin presented in real samples. Gold nanoparticles were electrodeposited at the surface of glassy carbon electrode in constant potential. Methods: The effect of several parameters such as time and potential of deposition, pH and scan rates on signal were studied. The cathodic peak current of Au³⁺ decreased with increasing amikacin concentration. Quantitative analysis of amikacin was performed using differential pulse voltammetry by following cathodic peak current of gold ions. Results: Two dynamic linear ranges of 1.0 × 10⁻⁸–1.0 × 10^-7 M and 5.0 × 10⁻⁷–1.0 × 10^-3 M were obtained and limit of detection was estimated 3.0× 10⁻⁹ M. Conclusion: The method was successfully determined amikacin in pharmaceutical preparation and human serum. The effect of several interference in determination of amikacin was also studied.