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Volume 13, Issue 4
  • ISSN: 2211-5501
  • E-ISSN: 2211-551X

Abstract

Background

Thaumatin-like proteins (TLPs) constitute the PR-5 family of plant pathogenesis-related (PR) proteins and are responsible for host defense and various growth and development-related processes. Mints ( sp.) are essential oil-bearing plants, mainly affected by water availability and fungal pathogens.

Objective

Identification of candidate TLPs in that may help to counter pathogen infection.

Methods

Several bioinformatics procedures were used to identify TLP gene sequences from and to characterize them for nucleotide length and composition, their translated proteins, subcellular location, signal peptides, post translational modifications, allergenicity potential, evolutionary relatedness, and miRNA targeting.

Results

19 TLP candidates were identified in (Mlo_TLP001 - Mlo_TLP0019) annotating the available transcriptome data. The molecular mass of Mlo_TLPs ranged between 35.43 kDa - 12.79 kDa. Expression analysis of the Mlo_TLPs revealed that Mlo_TLP003, 006, 008, 010, 012, 013, 014, and 018 were upregulated in stem as compared to the root under control conditions. Further, the analysis of control and V. dahliae infected plants has shown Mlo_TLP006 to be upregulated in both the infected stem and root tissues as compared to the control.

Conclusion

Mlo_TLP006 is a probable candidate that imparts resistance to fungal pathogens in .

© 2024 Bentham Science Publishers
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2024-09-16
2025-01-19

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References

  1. EbrahimS. Pathogenesis related (PR) proteins in plant defense mechanism.Sci. Against Microb. Pathog2011210431054
     [Google Scholar]
  2. JainD. Role of pathogenesis-related (PR) proteins in plant defense mechanism.Molecular aspects of plant-pathogen interactionSpringer2018
     [Google Scholar]
  3. de Jesús-PiresC. Ferreira-NetoJ.R.C. Pacifico Bezerra-NetoJ. KidoE.A. de Oliveira SilvaR.L. PandolfiV. Wanderley-NogueiraA.C. BinneckE. da CostaA.F. Pio-RibeiroG. Pereira-AndradeG. SittolinI.M. Freire-FilhoF. Benko-IsepponA.M. Plant thaumatin-like proteins: function, evolution and biotechnological applications.Curr. Protein Pept. Sci.2020211365110.2174/138920372066619031816490530887921
     [Google Scholar]
  4. van der WelH. LoeveK. Isolation and characterization of thaumatin I and II, the sweet-tasting proteins from Thaumatococcus daniellii Benth.Eur. J. Biochem.197231222122510.1111/j.1432‑1033.1972.tb02522.x4647176
     [Google Scholar]
  5. GrenierJ. PotvinC. AsselinA. Some fungi express β-1,3-glucanases similar to thaumatin-like proteins.Mycologia200092584184810.1080/00275514.2000.12061228
     [Google Scholar]
  6. SakamotoY. WatanabeH. NagaiM. NakadeK. TakahashiM. SatoT. Lentinula edodes tlg1 encodes a thaumatin-like protein that is involved in lentinan degradation and fruiting body senescence.Plant Physiol.2006141279380110.1104/pp.106.07667916648221
     [Google Scholar]
  7. LiuJ.J. SturrockR. EkramoddoullahA.K.M. The superfamily of thaumatin-like proteins: its origin, evolution, and expression towards biological function.Plant Cell Rep.201029541943610.1007/s00299‑010‑0826‑820204373
     [Google Scholar]
  8. GhoshR. ChakrabartiC. Crystal structure analysis of NP24-I: a thaumatin-like protein.Planta2008228588389010.1007/s00425‑008‑0790‑518651170
     [Google Scholar]
  9. LiZ. WangX. CuiY. QiaoK. ZhuL. FanS. MaQ. Comprehensive genome-wide analysis of thaumatin-like gene family in four cotton species and functional identification of GhTLP19 involved in regulating tolerance to Verticillium dahlia and drought.Front. Plant Sci.20201157501510.3389/fpls.2020.57501533193513
     [Google Scholar]
  10. PetreB. MajorI. RouhierN. DuplessisS. Genome-wide analysis of eukaryote thaumatin-like proteins (TLPs) with an emphasis on poplar.BMC Plant Biol.20111113310.1186/1471‑2229‑11‑3321324123
     [Google Scholar]
  11. KrebitzM. WagnerB. FerreiraF. PeterbauerC. CampilloN. WittyM. KolarichD. SteinkellnerH. ScheinerO. BreitenederH. Plant-based heterologous expression of Mal d 2, a thaumatin-like protein and allergen of apple (Malus domestica), and its characterization as an antifungal protein.J. Mol. Biol.2003329472173010.1016/S0022‑2836(03)00403‑012787673
     [Google Scholar]
  12. KalraA. SinghH.B. PandeyR. SamadA. PatraN.K. KumarS. Diseases in mint: causal organisms, distribution, and control measures.J. Herbs Spices Med. Plants2005111-2719110.1300/J044v11n01_03
     [Google Scholar]
  13. ZimowskaB.E. Fungi colonizing and damaging different parts of peppermint (Mentha piperita L.) cultivated in south-eastern Poland.Herba Pol.200753497105
     [Google Scholar]
  14. RamD. RamM. SinghR. Optimization of water and nitrogen application to menthol mint (Mentha arvensis L.) through sugarcane trash mulch in a sandy loam soil of semi-arid subtropical climate.Bioresour. Technol.200697788689310.1016/j.biortech.2005.04.04716005209
     [Google Scholar]
  15. ShorminT. KhanM. AlamgirM. Response of different levels of nitrogen fertilizer and water stress on the growth and yield of Japanese mint (Mentha arvensis L.).Bangladesh J. Sci. Indust. Res.200944113714510.1016/j.plaphy.2023.10824338048701
     [Google Scholar]
  16. ZhaoY. YangX. ZhangJ. HuangL. ShiZ. TianZ. ShaA. LuG. Thaumatin-like protein family genes VfTLP4-3 and VfTLP5 are critical for faba bean’s response to drought stress at the seedling stage.Plant Physiol. Biochem.202420610824310.1016/j.plaphy.2023.10824338048701
     [Google Scholar]
  17. HeL. LiL. ZhuY. PanY. ZhangX. HanX. LiM. ChenC. LiH. WangC. BolTLP1, a Thaumatin-like Protein Gene, Confers Tolerance to Salt and Drought Stresses in Broccoli (Brassica oleracea L. var. Italica).Int. J. Mol. Sci.202122201113210.3390/ijms22201113234681789
     [Google Scholar]
  18. MuokiR.C. PaulA. KaachraA. KumarS. Membrane localized thaumatin-like protein from tea (CsTLP) enhanced seed yield and the plant survival under drought stress in Arabidopsis thaliana. Plant Physiol. Biochem.2021163364410.1016/j.plaphy.2021.03.01233812225
     [Google Scholar]
  19. LiuY. CuiJ. ZhouX. Genome-wide identification, characterization and expression analysis of the TLP gene family in melon (Cucumis melo L.).Genomics2020112324992509
     [Google Scholar]
  20. RatherI.A. AwasthiP. MahajanV. BediY.S. VishwakarmaR.A. GandhiS.G. Molecular cloning and functional characterization of an antifungal PR-5 protein from Ocimum basilicum. Gene2015558114315110.1016/j.gene.2014.12.05525550044
     [Google Scholar]
  21. NawrotR. MusidlakO. BarylskiJ. NowickiG. BałdyszS. CzerwoniecA. Goździcka-JózefiakA. Characterization and expression of a novel thaumatin-like protein (CcTLP1) from papaveraceous plant Corydalis cava. Int. J. Biol. Macromol.202118967868910.1016/j.ijbiomac.2021.08.06734390750
     [Google Scholar]
  22. SamalK.C. RoutG.R. Computational analyses of allergens for improved allergenicity risk assessment in Bt brinjal (Solanum melongena).Proceedings of the National Seminar on Plant Genomics and Biotechnology: Challenges and Opportunities in 21st CenturyExcel India Publishers, New Delhi.2016
     [Google Scholar]
  23. YanX. QiaoH. ZhangX. GuoC. WangM. WangY. WangX. Analysis of the grape (Vitis vinifera L.) thaumatin-like protein (TLP) gene family and demonstration that TLP29 contributes to disease resistance.Sci. Rep.201771426910.1038/s41598‑017‑04105‑w28655869
     [Google Scholar]
  24. IqbalI. TripathiR.K. WilkinsO. SinghJ. Thaumatin-like protein (TLP) gene family in barley: Genome-wide exploration and expression analysis during germination.Genes (Basel)2020119108010.3390/genes1109108032947963
     [Google Scholar]
  25. SaeidiM. ZareieR. Prediction, isolation, overexpression and antifungal activity analysis of Medicagotruncatula var. truncatula putative thaumatin like proteins (TLP-1, -2, -3, -4 and -5).Turk. J. Biol.202044417618710.3906/biy‑1912‑1832922125
     [Google Scholar]
  26. SuL. ZhaoX. GengL. Analysis of the thaumatin-like genes of Rosa chinensis and functional analysis of the role of RcTLP 6 in salt stress tolerance.Planta202125415
     [Google Scholar]
  27. WangX. ZafianP. ChoudharyM. LawtonM. The PR5K receptor protein kinase from Arabidopsis thaliana is structurally related to a family of plant defense proteins.Proc. Natl. Acad. Sci. USA19969362598260210.1073/pnas.93.6.25988637920
     [Google Scholar]
  28. AnžlovarS. DermastiaM. The comparative analysis of osmotins and osmotin-like PR-5 proteins.Plant Biol.20035211612410.1055/s‑2003‑40723
     [Google Scholar]
  29. ZhangY. YanH. WeiX. ZhangJ. WangH. LiuD. Expression analysis and functional characterization of a pathogen-induced thaumatin-like gene in wheat conferring enhanced resistance to Puccinia triticina.J. Plant Interact.201712133233910.1080/17429145.2017.1367042
     [Google Scholar]
  30. YinJ. YiH. ChenX. WangJ. Post-translational modifications of proteins have versatile roles in regulating plant immune responses.Int. J. Mol. Sci.20192011280710.3390/ijms2011280731181758
     [Google Scholar]
  31. YangM. Role of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichia pastoris.Microb. Cell Fact.20151414
     [Google Scholar]
  32. BreitenederH. Thaumatin-like proteins–a new family of pollen and fruit allergens.Allergy2002595479481
     [Google Scholar]
  33. InschlagC. Hoffmann-SommergruberK. O’RiordainG. AhornH. EbnerC. ScheinerO. BreitenederH. Biochemical characterization of Pru a 2, a 23-kD thaumatin-like protein representing a potential major allergen in cherry (Prunus avium).Int. Arch. Allergy Immunol.19981161222810.1159/0000239209623505
     [Google Scholar]
  34. Jensen-JarolimE. SantnerB. LeitnerA. GrimmR. ScheinerO. EbnerC. BreitenederH. Bell peppers (Capsicum annuum) express allergens (profilin, pathogenesis-related protein P23 and Bet v 1) depending on the horticultural strain.Int. Arch. Allergy Immunol.1998116210310910.1159/0000239329652302
     [Google Scholar]
  35. SassaH. HiranoH. Style-specific and developmentally regulated accumulation of a glycosylated thaumatin/PR5-like protein in Japanese pear ( Pyrus serotina Rehd.).Planta1998205451452110.1007/s0042500503509684355
     [Google Scholar]
  36. Van DammeE. CharelsD. Menu-BouaouicheL. ProostP. BarreA. RougéP. PeumansW. Biochemical, molecular and structural analysis of multiple thaumatin-like proteins from the elderberry tree ( Sambucus nigra L.).Planta2002214685386210.1007/s00425‑001‑0713‑111941461
     [Google Scholar]
  37. KimY.S. ParkJ.Y. KimK.S. KoM.K. CheongS.J. OhB.J. A thaumatin-like gene in nonclimacteric pepper fruits used as molecular marker in probing disease resistance, ripening, and sugar accumulation.Plant Mol. Biol.200249212513510.1023/A:101499573217111999369
     [Google Scholar]
  38. PiggottN. EkramoddoullahA.K.M. LiuJ.J. YuX. Gene cloning of a thaumatin-like (PR-5) protein of western white pine (Pinus monticola D. Don) and expression studies of members of the PR-5 group.Physiol. Mol. Plant Pathol.20046411810.1016/j.pmpp.2004.05.004
     [Google Scholar]
  39. TachiH. Fukuda-YamadaK. KojimaT. ShiraiwaM. TakaharaH. Molecular characterization of a novel soybean gene encoding a neutral PR-5 protein induced by high-salt stress.Plant Physiol. Biochem.2009471737910.1016/j.plaphy.2008.09.01219010689
     [Google Scholar]
  40. MunisM.F.H. TuL. DengF. TanJ. XuL. XuS. LongL. ZhangX. A thaumatin-like protein gene involved in cotton fiber secondary cell wall development enhances resistance against Verticillium dahliae and other stresses in transgenic tobacco.Biochem. Biophys. Res. Commun.20103931384410.1016/j.bbrc.2010.01.06920097164
     [Google Scholar]
  41. SunW. ZhouY. MovahediA. WeiH. ZhugeQ. Thaumatin-like protein(Pe-TLP)acts as a positive factor in transgenic poplars enhanced resistance to spots disease.Physiol. Mol. Plant Pathol.202011210151210.1016/j.pmpp.2020.101512
     [Google Scholar]
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Transcriptome Mining, Identification and In silico Characterization of Thaumatin-Like Protein Sequences from Mentha Longifolia
Curr. Biotechnol. 13, 195 (2024); https://doi.org/10.2174/0122115501319317240903112811
/content/journals/cbiot/10.2174/0122115501319317240903112811
/content/journals/cbiot/10.2174/0122115501319317240903112811
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Supplements

Supplementary File 1. Prediction of the allergenic potential of Mlo_TLPs. Supplementary File 2. Prediction of miRNAs that target the Mlo_TLPs. Supplementary material is available on the publisher’s website along with the published article.

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  • Article Type:     Research Article
Keyword(s): bioinformatics; expression analysis, pathogenesis; mentha longifolia; PR proteins; Thaumatin-like proteins

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