Database Searching in Mass Spectrometry Based Proteomics

Attila Kertesz-Farkas; Beata Reiz; Michael P. Myers; Sandor Pongor

doi:10.2174/157489312800604354

ISSN: 1574-8936
E-ISSN: 2212-392X

Database Searching in Mass Spectrometry Based Proteomics
Authors: Attila Kertesz-Farkas¹, Beata Reiz², Michael P. Myers³ and Sandor Pongor⁴
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Affiliations: ¹ Protein Structure and Bioinformatics Group, International Centre for Genetic Engineering and Biotechnology, 34012 Trieste, Italy. ² Protein Structure and Bioinformatics Group, International Centre for Genetic Engineering and Biotechnology, 34012 Trieste, Italy. ³ Protein Structure and Bioinformatics Group, International Centre for Genetic Engineering and Biotechnology, 34012 Trieste, Italy. ⁴ Protein Structure and Bioinformatics Group, International Centre for Genetic Engineering and Biotechnology, 34012 Trieste, Italy.
Source: Current Bioinformatics, Volume 7, Issue 2, Jun 2012, p. 221 - 230
DOI: https://doi.org/10.2174/157489312800604354
- Available online: 01 Jun 2012

Abstract

Bottom-up proteomics (mass spectrometry analysis of peptides obtained by proteolysis and separated by liquid chromatography, (LC-MS/MS)) is one of the most frequently used techniques for identifying and characterizing proteins in biological samples. A key element of the analysis is database searching when the mass spectra of the peptides are compared with a database of theoretically computed (or experimental) peptide spectra. Here we discuss the main computational approaches to spectrum database searching and the statistical analysis of the results.

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2012-06-01

2025-01-29

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Article Type: Research Article

Keyword(s): 3D structure analysis); Database search; fragment; genome annotation; Liquid chromatography; Mass spectrometry; mass spectrum; pattern recognition; Protein identification; Proteomics

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Database Searching in Mass Spectrometry Based Proteomics

Abstract

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